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In vitro synthesis of biologically active transcripts of tomato b...
Synthetic transcripts of tomato black ring virus satellite RNA (TBRV satRNA), isolate L, were prepared from cDNA cloned in the Bluescribe transcription vector. Transcripts with 49 (T49L) or two (T2GL)...
A gene trap vector system for identifying transcriptionally respo...
We present a method for fast and efficient trapping of genes whose transcription is regulated by exogenous stimuli. We constructed a promoterless retroviral vector transducing a green fluorescent prot...
Quantitation and integrity evaluation of RNA genome in lentiviral...
Lentiviral vectors (LV) have proven to be powerful tools for stable gene delivery in both dividing and non-dividing cells. Approval of these LVs for use in clinical applications has been achieved by i...
How to generate high-impact data in AAV gene therapy testing | Al...
How do you generate high-impact data in preclinical testing of AAV gene therapy?
In this case study, see how our team used fully validated ddPCR and RT-ddPCR methods to precisely quantify vector and...
Establishing glucose- and ABA-regulated transcription networks in...
Establishing transcriptional regulatory networks by analysis of gene expression data and promoter sequences shows great promise. We developed a novel promoter classification method using a Relevance V...
Symptom Production on Tobacco and Tomato Is Determined by Two Dis...
SUMMARY
Complementary DNAs of two different satellite RNA isolates from cucumber mosaic virus (CMV), Y from Japan and Ra from France, have been cloned in a transcription vector containing the Pr promo...
Production of phenocopies by Krüppel antisense RNA injection into...
The demonstration that a specific messenger RNA can he functionally inactivated in vivo hy hybridization to complementary polynucleotide sequences suggests a direct approach to the study of gene funct...
YouTube's Ask AI Teardown: Gen-AI for Interactive Knowledge | Aar...
Visual Gen-AI Product Teardown: Inside YouTube Ask AI
Built a visual teardown of YouTube’s Ask AI to understand how Gen-AI is transforming video platforms.
Key insight: Users don’t want videos. They...
Production of phenocopies by Krüppel antisense RNA injection into...
The demonstration that a specific messenger RNA can he functionally inactivated in vivo hy hybridization to complementary polynucleotide sequences suggests a direct approach to the study of gene funct...
Trippi 2.0 Architecture: Real-Time Agentic RAG System with FastAP...
Trippi 2.0 Architecture Reveal 🏗️
Building a real-time Agentic RAG system meant solving for data gaps and latency. Here is how Trippi handles it:
Streaming Core: FastAPI + Asynchronous StreamingRespon...
Generation of functional neurons from adult human mucosal olfacto...
A recent approach to promote central nervous system (CNS) regeneration after injury or disease is direct conversion of somatic cells to neurons. This is achieved by transduction of viral vectors that ...
Phage Vectors that Allow Monitoring of Transcription of Secondary...
We describe a bacteriophage øC31-based system that permits the transcriptional fusion of the convenient reporter gene xylE to chromosomally located promoters in Streptomyces hosts. Applicability of th...
A support vector machine model provides an accurate transcript-le...
Major depressive disorder (MDD) is a critical cause of morbidity and disability with an economic cost of hundreds of billions of dollars each year, necessitating more effective treatment strategies an...
A support vector machine model provides an accurate transcript-le...
Major depressive disorder (MDD) is a critical cause of morbidity and disability with an economic cost of hundreds of billions of dollars each year, necessitating more effective treatment strategies an...
A support vector machine model provides an accurate transcript-le...
Major depressive disorder (MDD) is a critical cause of morbidity and disability with an economic cost of hundreds of billions of dollars each year, necessitating more effective treatment strategies an...
Unified Multimodal Search Stack with Gemini 2 and Qdrant | Mohamm...
One #Model to Find Them All (and it's 4x cheaper to store)
Stop building three different #pipelines for text, images, and audio. It’s a DevOps nightmare and a data integrity trap
This deep dive explor...
Removal of evolutionarily conserved functional MYC domains in a t...
MYC transcription factors have critical roles in facilitating a variety of cellular functions that have been highly conserved among species during evolution. However, despite circumstantial evidence f...
Direct conversion of porcine primary fibroblasts into hepatocyte-...
The pig is an important model organism for biomedical research, mainly due to its extensive genetic, physiological and anatomical similarities with humans. Until date, direct conversion of somatic cel...
A lentivirus-based system to functionally silence genes in primar...
RNA interference (RNAi) has recently emerged as a specific and efficient method to silence gene expression in mammalian cells either by transfection of short interfering RNAs (siRNAs; ref. 1) or, more...
Positive-negative selection gene targeting with the diphtheria to...
The diphtheria toxin A-chain gene was used in a positive-negative selection gene targeting vector to alter the CD4 gene which is transcriptionally silent i